In fluorometry, the intensity of emitted light is proportional to:

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Multiple Choice

In fluorometry, the intensity of emitted light is proportional to:

In fluorometry, the emitted light scales with how many fluorescent molecules are present to emit photons after being excited. If the excitation conditions and the fluorophore’s ability to emit (quantum yield) are effectively constant, the intensity of the emitted light is proportional to the concentration of the fluorescent compound that responds at the detected wavelength. The solvent, instrument gain, or temperature can influence the signal by changing detection efficiency or the fluorophore’s quantum yield, but the basic, direct relationship is with the fluorophore’s concentration (within the linear, non-saturated range).

In fluorometry, the emitted light scales with how many fluorescent molecules are present to emit photons after being excited. If the excitation conditions and the fluorophore’s ability to emit (quantum yield) are effectively constant, the intensity of the emitted light is proportional to the concentration of the fluorescent compound that responds at the detected wavelength. The solvent, instrument gain, or temperature can influence the signal by changing detection efficiency or the fluorophore’s quantum yield, but the basic, direct relationship is with the fluorophore’s concentration (within the linear, non-saturated range).

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